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KMID : 0903619920330050379
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Journal of the Korean Society for Horticultural Science
1992 Volume.33 No. 5 p.379 ~ p.383
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Characterization of β - Galactosidase from ´ Fuji ´ Apple Fruits
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Abstract
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β-Galactosidase was separated from ´Fuji´ apple fruits by gel filtration, ion exchange column chromatography and characterized by means of several biochemical methods. The results obtained are summarized as follows:
1. One form of β-galactosidase was detected.
2. The Km and Vmax values of β-galactosidase were 3.5 × 10^(-2) mM and 3.6 × 10^(-2) mmole ρ-nitrophenyl-β-D-galactopyranoside/㎖/15 min, respectively.
3. The β-galactosidase was stable below 45℃ with the highest activity at 35℃.
4. The β-galactosidase was stable at pH between 3 and 7 with the highest activity at pH between 4 and 5.
5. The β-galactosidase activity was inhibited by Ag^+, SDS, or EDTA.
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